Human Anti-DPP4 Recombinant Antibody (HPAB-0215-YC) (CAT#: HPAB-0215-YC)
This product is a recombinant humanized antibody that recognizes DPP4. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography. The anti-proliferative effect of specified recombinant human MAb was analyzed in Jurkat CD26+ cells and the most inhibitory concentration was at 5 μg/mL. The antibody has an a discontinuous or conformational epitope. It had high reactivity against peptides: YSLRWISDHEYLY, YVKQWRHSYTASY, TWSPVGHKLAYVW, EEEVFSAYSALWW and RISLQWLRRIQNY.
Specific Inquiry
WB
Figure 1 Anti-DPP4 Recombinant Antibody (HPAB-0215-YC) in WB
Western blot analysis of HPAB-0215-YC (2 μg/mL) was performed with Recombinant Human DPP4/CD26 Protein (His Tag). The HRP-Anti-Human IgG (H+L) (1: 6000) was used as a secondary antibody.
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg
DB
Figure 2 Anti-DPP4 Recombinant Antibody (HPAB-0215-YC) in DB
Dot Blot analysis of HPAB-0215-YC (2 μg/mL) was performed by coating with Recombinant Human DPP4/CD26 Protein (His Tag). The HRP-Anti-Human IgG (H+L) (1: 6000) was used as a secondary antibody.
ELISA
Figure 3 Anti-DPP4 Recombinant Antibody (HPAB-0215-YC) in ELISA
ELISA analysis of HPAB-0215-YC was performed by coating with Recombinant Human DPP4/CD26 Protein (His Tag). Then blocked with BSA, and incubated with HPAB-0215-YC. The HRP-conjugated goat Anti-Human IgG was used as a secondary antibody (1:5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG1
- Specificity
- Human DPP4
- Species Reactivity
- Human
- Applications
- ELISA, Inhib, FuncS
- Related Disease
- Diseases associated with expression of CD26
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Dipeptidyl-Peptidase 4; Adenosine Deaminase Complexing Protein 2; Dipeptidylpeptidase IV (CD26, Adenosine Deaminase Complexing Protein 2); T-Cell Activation Antigen CD26; Dipeptidyl Peptidase IV; EC 3.4.14.5; DPP IV; ADCP-2; ADCP2; ADABP; TP103; CD26; Dipeptidylpeptidase 4; CD26 Antigen; DPPIV
- Gene ID
- 1803
- UniProt ID
- P27487
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Flow Cytometry (FC) related Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-316 | Mouse Anti-Dpp4 Recombinant Antibody (clone 11A19) | ELISA, WB, FuncS | Mouse IgG |
| PABL-690 | Mouse Anti-Dpp4 Recombinant Antibody (clone MK-0431) | WB, FuncS | Mouse IgG |
| HPAB-0213-YC | Mouse Anti-DPP4 Recombinant Antibody (HPAB-0213-YC) | FC, FuncS | Mouse IgG2b |
| MRO-0290-CN | Rabbit Anti-DPP4 Recombinant Antibody (clone JM11-42) | WB, IF, IHC, FC | Rabbit IgG |
| HPAB-3029LY | Human Anti-DPP4 Recombinant Antibody (HPAB-3029LY) | FC | Human IgG |
Customer Reviews and Q&As
Customer Review
Q&As
Background That Is Not Reactive
The antibody appears to have produced an excellent non-reactive background. It guarantees that the information gathered is precise and unique to the target protein.
Simple to Follow Procedures
The antibody comes with user-friendly, easy-to-follow methods that guarantee successful experimental outcomes without requiring a lot of debugging.
Reliable for Prolonged Research
Long-term investigations have shown that this antibody is quite dependable, performing consistently throughout time. It's a stable and reliable product.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0215-YC, RRID: AB_3111372)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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